This book demonstrates the extremely fascinating and interdisciplinary microbial domain. It helps in discovering the latest advances in microbiology and learns how they can help shape a more sustainable future for our planet. This comprehensive guide covers the latest breakthroughs in microbiology research and their practical applications in fields such as ecology, agriculture, biotechnology, and environmental science. Furthermore, the readers explore the cutting-edge technologies and methodologies that are driving the next generation of microbiology research. With expert insights from the leading microbiologists, this book is an essential resource for anyone interested in understanding the role of microbes in our world and harnessing their power for a better tomorrow.

Medical laboratories not only assist the physicians in diagnosis and management of a disease but also play a role in community health. Apart from the analytical aspects of various investigations, the larger areas of human resource management, inventory management and the like have been gaining importance as a part of laboratory management. There is a felt need for an understanding of the managerial aspects of a clinical laboratory, especially in an institutional setting. Although this knowledge can be acquired from books on general management subject, there is a paucity of books dedicated to clinical laboratory management. Hence is a need for a simple and concise text for management in the clinical laboratory practice.

This book contains chapters on wearable biomedical sensors and their assistive technologies for promoting behavioral change in medical and health care. Part I reviews several wearable biomedical sensors based on biocompatible materials and nano and micro-electromechanical systems (MEMS) technologies in the medical and dental fields. Part II introduces the latest approaches to wearable biosensing using unique devices for various skin targets such as sweat, interstitial fluid, and transcutaneous gases. Part III presents technologies supporting wearable sensors, including soft and flexible materials, manufacturing methods, skin volatile-marker imaging, and energy harvesting devices.

This book highlights key findings generated during the past years from the main disciplines that constitute Physical Virology, from theoretical physics and simulations to material sciences and vaccines development to structural biology. Each chapter is written by world-class scientists from these areas and is a comprehensive review of where this field stands, as well as the future of Physical Virology. The diversity in the formal training of these scientists results in solving common problems using very distinct approaches, which can produce surprising findings. The multi- and interdisciplinary nature of this field has created a remarkable community that aims at understanding how viruses work and how they can be used in material sciences, chemistry, and biomedicine. Furthermore, the development of Physical Virology has resulted in technological advances that have shaped other fields; for example, it would be impossible to think about the development of Cryo-EM to solve the structure of complex viruses with atomic resolution without the contribution of scientists that created the field of Physical Virology. In the past decade, there has been a great success in the generation of viral systems that can encapsulate drugs, non-viral genetic material, or nanoparticles, as well as in the chemical and genetical modification of virions. Without any doubt in the immediate future, some of these technologies will jump from the bench to the market, creating a revolution in translational and biomedical sciences. The book provides key perspectives for the field, derived from expert´s opinions.

This book chronicles the role of advanced nanomaterials and surface engineering technologies in the development of point-of-care biosensors for health and environmental monitoring. All aspects of nanomaterial synthesis and characterization, functionalization methods, sensing surface engineering, signal amplification strategies, use of innovative technologies to enhance sensor efficiency and performances, and innovative applications of nanobiosensors to tackle real-life problems are discussed in this book with a focus on optical and electrochemical based sensing. It also covers the detection of infectious diseases and various disease biomarkers, smartphone-based biosensing, and portable diagnostics module developments with a discussion on the working mechanisms of these devices in various domains. The book also illustrates the recent trends in biosensing, and an overview of the challenges and probable solutions for the translation of biosensors from laboratory prototypes to commercial success.

This classroom-tested textbook is an innovative, comprehensive, and forward-looking introductory undergraduate physics course. While it clearly explains physical principles and equips the student with a full range of quantitative tools and methods, the material is firmly grounded in biological relevance and is brought to life with plenty of biological examples throughout.

This book is the first comprehensive compilation of existing knowledge on the impact of cisgenic crops on biodiversity, environment, and ecosystem. Strategies to create and access cisgenic crops, potential risks assessment, and legal implications across the globe (European Union, North and South Americas, Africa, and Asia) are enumerated. Ethics, economics, safety, social concerns, and consumer acceptance of cisgenic foods are deliberated. Policy paradigm and ways to overcome GM regulations through cisgenic crops are highlighted. The book has 12 chapters authored by internationally leading experts on the subject. This book will be useful especially for the policy makers and GMO regulators, while the students, teachers, and researchers from across the globe will be equally benefited.

The field of nanotechnology for targeted therapy initiated more than decade ago has grown fast and interest is increasing. Given the importance of the field for targeted drug and gene delivery systems, there are a large number of laboratory investigations today researching nanobiomaterials for diagnostic and therapeutic applications. Because of the ability of scientists to load nanoparticles with any agent, interest continues to grow and technology in this arena is rapidly evolving. These emerging nanobiomaterials-based medicines can overcome the disadvantages of traditional medicines by target-oriented and site-specific delivery of precise medicines (immunotherapeutic agents, chemotherapeutic agents, diagnostic agents, and so on).

This book offers an excellent introduction to Korean functional foods and shares latest important information for food scientists and nutritionists, including accurate, up-to-date information on Korean food science together with background information, archeological findings, as well as food methods and research on Korean fermented foods (e.g., grain wine, kimchi, jeotgal, and soybean sauces). It also discusses historical backgrounds and manufacturing method details of traditional food categories, such as rice cakes, sweets, fermented sauces, and alcoholic and non-alcoholic beverages, and helps us understand the full science behind Korean traditional food.

This book compiles the relevant information related to genome editing tools and their roles in crop improvement. The book contains a brief introduction about various genome editing tools and their application in major crops. It discusses the genome editing approaches and the strategies used for genome editing in different crops. Some of the chapters cover the detailed methodology of sgRNA design, vector construction and transformation in different crops followed by data analysis. A few chapters focus on the applications of genome editing tools towards crop improvement. This book will be of particular interest to plant biologists working in the field of genome editing and crop breeders. It will provide valuable information and useful material for our readers’ experimental work.

Understanding the functional roles of DNA modifications relies on the accurate detection, quantification, and mapping of DNA modifications. Methods for deciphering DNA modifications have substantially improved over the last several years, which greatly revolutionize the field of DNA modifications. In addition to DNA cytosine methylation (5-methylcytosine, 5mC), the best-characterized epigenetic modification, many new modifications have been discovered to present in DNA in recent years.

This volume details methods on several aspects of circadian research. Chapters guide readers through the latest techniques and a wide variety of daily rhythmic processes, model organisms, circadian rhythms in the SCN and in peripheral organs, and describing in vitro systems and in silico methods. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.
Authoritative and cutting-edge, Circadian Regulation: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field.

Understanding the functional roles of DNA modifications relies on the accurate detection, quantification, and mapping of DNA modifications. Methods for deciphering DNA modifications have substantially improved over the last several years, which greatly revolutionize the field of DNA modifications. In addition to DNA cytosine methylation (5-methylcytosine, 5mC), the best-characterized epigenetic modification, many new modifications have been discovered to present in DNA in recent years.

This comprehensive guide to gene cloning provides beginning and advanced readers with the background, standard techniques, practical applications, and ethical and safety considerations in the field. A one-stop reference for students, researchers in academia and industry, and anyone interested in a thorough but accessible overview.

This volume describes methods and protocols for the fragment-based screening of proteins using Surface Plasmon Resonance (SPR). The initial chapter of the book discusses the principle of SPR for the identification of biomolecular interactions, while the subsequent chapters introduce methods for labelling proteins with different tags including, histidine and biotin tags.