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  3. Protein Biochemistry

The Proteomics Protocols Handbook

Posted By: AvaxGenius
Date: 17 Mar 2023 14:28:40
The Proteomics Protocols Handbook

The Proteomics Protocols Handbook by John M. Walker
English | PDF (True) | 2005 | 969 Pages | ISBN : 1588293432 | 15.06 MB

Hands-on researchers describe in step-by-step detail 73 proven laboratory methods and bioinformatics tools essential for analysis of the proteome. These cutting-edge techniques address such important tasks as sample preparation, 2D-PAGE, gel staining, mass spectrometry, and post-translational modification. There are also readily reproducible methods for protein expression profiling, identifying protein-protein interactions, and protein chip technology, as well as a range of newly developed methodologies for determining the structure and function of a protein. The bioinformatics tools include those for analyzing 2D-GEL patterns, protein modeling, and protein identification. All laboratory-based protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
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Fractal Symmetry of Protein Interior

Posted By: AvaxGenius
Date: 10 Dec 2022 07:12:19
Fractal Symmetry of Protein Interior

Fractal Symmetry of Protein Interior by Anirban Banerji
English | PDF | 2013 | 92 Pages | ISBN : 3034806507 | 2.1 MB

The essential question that fractal dimensions attempt to answer is about the scales in Nature. For a system as non-idealistic and complex as a protein, studying scale-invariance becomes particularly important.
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Therapeutic Proteins Against Human Diseases

Posted By: AvaxGenius
Date: 2 Oct 2022 22:18:21
Therapeutic Proteins Against Human Diseases

Therapeutic Proteins Against Human Diseases by Fahmida Khatoon Zahid Balouch
English | PDF,EPUB | 2022 | 178 Pages | ISBN : 9811678960 | 19.9 MB

This book compiles updated research about the implications of therapeutic proteins in various human diseases. The initial chapters of the book provide basic information on the therapeutic proteins and discuss techniques for their formulation, production, and analytic approaches for their characterization. The subsequent chapters shed light on therapies based on therapeutic proteins against metabolic disorders, neurological disorders, cancer, autoimmune disorders, and infectious diseases. Importantly, it presents the factors influencing the immunogenicity of therapeutic proteins, including, genetic factors, disease type, and origin of therapeutic protein, dose frequency, administration route, and treatment duration.
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Carbonic Anhydrase: Mechanism, Regulation, Links to Disease, and Industrial Applications (Repost)

Posted By: AvaxGenius
Date: 7 Aug 2022 15:46:02
Carbonic Anhydrase: Mechanism, Regulation, Links to Disease, and Industrial Applications (Repost)

Carbonic Anhydrase: Mechanism, Regulation, Links to Disease, and Industrial Applications by Susan C. Frost, Robert McKenna
English | PDF | 2014 | 429 Pages | ISBN : 9400773587 | 8 MB

The study of carbonic anhydrase has spanned multiple generations of scientists. Carbonic anhydrase was first discovered in 1932 by Meldrum and Roughton. Inhibition by sulfanilamide was shown in 1940 by Mann and Keilin. Even Hans Krebs contributed to early studies with a paper in 1948 showing the relationship of 25 different sulfonamides to CA inhibition. It was he who pointed out the importance of both the charged and uncharged character of these compounds for physiological experiments.
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piRNA: Methods and Protocols

Posted By: AvaxGenius
Date: 11 Jul 2022 03:56:55
piRNA: Methods and Protocols

piRNA: Methods and Protocols by Nicholas F. Parrish
English | PDF | 2022 | 396 Pages | ISBN : 1071623796 | 11.3 MB

This detailed volume explores newly-developed methods in PIWI-interacting RNAs (piRNAs) research, methods currently applied to other ncRNAs involved in nuclear regulation which can be used to study piRNAs, and piRNA methods applied in non-classical organisms. It also includes several bioinformatic and biophysical methods related to piRNA studies, consistent with the increasing importance of high-throughput sequencing and computational methods. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.
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Effector-Triggered Immunity: Methods and Protocols

Posted By: AvaxGenius
Date: 2 Jul 2022 15:20:28
Effector-Triggered Immunity: Methods and Protocols

Effector-Triggered Immunity: Methods and Protocols by Thomas A. Kufer
English | PDF | 2022 | 316 Pages | ISBN : 1071624482 | 8 MB

This volume presents a collection of protocols to study effector-triggered immunity (ETI) in both plants and animals from eminent groups in the field. The chapters in this book cover topics such as genetic manipulation of plant and animal pathogens, host cells, and the analysis of key host responses; and techniques used for the analysis of inflammasome activation, cell death pathways, and mitochondria damage in response to pathogens. All of these topics cover a broad spectrum of immunological, biochemical, cell biological, and structural biology approaches to examine ETI. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
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Advances in MALDI and Laser-Induced Soft Ionization Mass Spectrometry

Posted By: AvaxGenius
Date: 15 May 2022 15:26:25
Advances in MALDI and Laser-Induced Soft Ionization Mass Spectrometry

Advances in MALDI and Laser-Induced Soft Ionization Mass Spectrometry by Rainer Cramer
English | PDF | 2016 | 287 Pages | ISBN : 331904818X | 8.9 MB

This book covers the state-of-the-art of modern MALDI (matrix-assisted laser desorption/ionization) and its applications. New applications and improvements in the MALDI field such as biotyping, clinical diagnosis, forensic imaging, and ESI-like ion production are covered in detail. Additional topics include MS imaging, biotyping/speciation; large-scale, high-speed MS sample profiling; new methods based on MALDI or MALDI-like sample preparations; and the advantages of MALDI MS analysis over ESI (electrospray ionization). This is an ideal book for graduate students and researchers in the field of bioanalytical sciences.
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Split Inteins: Methods and Protocols

Posted By: AvaxGenius
Date: 12 May 2022 14:17:53
Split Inteins: Methods and Protocols

Split Inteins: Methods and Protocols by Henning D. Mootz
English | PDF | 2017 | 275 Pages | ISBN : 1493964496 | 8.8 MB

This volume focuses on applications of split inteins, and the progress that has been made in the past 5 years on discovery and engineering of fast and more efficient split inteins. The first few chapters in Split Inteins: Methods and Protocols explore new techniques on how to use split inteins for affinity purification of overproduced proteins, and split-intein based technologies to prepare cyclic peptides and proteins.
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CCN Proteins: Methods and Protocols

Posted By: AvaxGenius
Date: 12 May 2022 14:11:18
CCN Proteins: Methods and Protocols

CCN Proteins: Methods and Protocols by Masaharu Takigawa
English | PDF | 2017 | 561 Pages | ISBN : 1493964283 | 19.7 MB

This volume provides a comprehensive guide on the Cyr61 (Cysteine-rich 61) (CCN) family of proteins and genes from basic research to cutting-edge methodologies and state-of-the-art techniques. Chapters details practical tips to overcome any obstacles with experimentation pertaining to chemistry, biology, physiology, pathology, medical and dental sciences and pharmacology of CCN proteins.
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Prediction of Protein Secondary Structure

Posted By: AvaxGenius
Date: 12 May 2022 14:04:11
Prediction of Protein Secondary Structure

Prediction of Protein Secondary Structure by Yaoqi Zhou
English | PDF | 2017 | 315 Pages | ISBN : 1493964046 | 12.1 MB

This thorough volume explores predicting one-dimensional functional properties, functional sites in particular, from protein sequences, an area which is getting more and more attention. Beginning with secondary structure prediction based on sequence only, the book continues by exploring secondary structure prediction based on evolution information, prediction of solvent accessible surface areas and backbone torsion angles, model building, global structural properties, functional properties, as well as visualizing interior and protruding regions in proteins. Written for the highly successful Methods in Molecular Biology series, the chapters include the kind of detail and implementation advice to ensure success in the laboratory.
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